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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Effects of ? -amylase Inhibitor on Mungbean Weevil, Callosobruchus maculatus, in vivo and in vitro and on Barley Malt ?-amylase in vitro) ผู้เขียน: อิทธิพล บรรณาการ,  ดร.ประภารัจ หอมจันทน์, ศาสตราจารย์ , นางอรุณี วงศ์ปิยะสถิตย์, ศาสตราจารย์ , Arunee Engkakul สื่อสิ่งพิมพ์:pdf AbstractEffects of protein part of ?-amylase inhibitor crude extracts from mungbean seeds of the recommended varieties, KPS1 and CN36, and mutant lines, M5-16 and M5-29 were evaluated on the mortality of the larval and adult stages of mungbean weevil, Callosobruchus maculatus. KPS1 seeds were used as the medium soaked with different extract concentrations of all mungbean varieties/lines and distilled water (the control). Each, with three replicates, was fed to one pair of C. maculatus and percent mortality of each stage was recorded. Of all different protein ?-amylase inhibitor extracts at each concentration, significant difference was not found in larval mortality percentage between the extract-treated seeds and distilled water-treated seeds. Only seeds treated with 0.2% w/w protein extracts from CN36 and KPS1 seeds significantly differed in percent adult mortality from those of the control, respectively. At 0.4, 0.6 and 1% w/w protein, each extract was significantly different from the control in adult mortality percentage. Each extract of various concentrations, except for KPS1 and M5-29 extracts gave significantly different larval mortality percentages at 0.4 and 0.6 and 1% w/w protein, respectively. There were significant differences in adult mortality percentage of every extract at each concentration from that of the control. An effect of ?-amylase inhibitor on ?-amylase activity in vitro was also studied. One hundred percent inhibition of protein part to ?-amylase activity of C. maculatus was found in all variety/line mungbean seeds. Similar study was also conducted against ? -amylase extracted from barley. The effects were less than those of ?-amylase of the weevil. |
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ที่มา:การประชุมทางวิชาการของมหาวิทยาลัยเกษตรศาสตร์ ครั้งที่ 46หัวเรื่อง:โปรตีโอมิกและคุณลักษณะของ alpha-amylase inhibitor จากถั่วเขียว |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Proteomic and Characterization of ?-amylase Inhibitor from Mungbean (Vigna radiate)) ผู้เขียน:อนุสสรณ์ วิเศษสิงห์, ดร.อรุณี อิงคากุล, ผู้ช่วยศาสตราจารย์, นางอรุณี วงศ์ปิยะสถิตย์, ศาสตราจารย์, Kiattawee Chuwongkomon สื่อสิ่งพิมพ์:pdf AbstractCallosobruchus maculatus, the insect pest, causes damaging to stored mungbean seeds leading to economical losses. For this reason the use of ?-amylase inhibitors to interfere the pest digestion process has been an interesting alternative as a biocontrolling agent. The studies of gamma irradiated mungbean mutant, M5-16, demonstrated that ?-amylase inhibitor from the mutant line was more inhibitory effective on C. maculatus ?-amylase than its wild type, KPS1. To investigate this mechanism, both proteomics and biochemical characterization were employed. The ?-amylase inhibitor from mungbean seed, M5-16, was purified in a two-step procedure involving ammonium sulfate precipitation and gel filtration chromatography using a sephadex G-100 column. This ?-amylase inhibitor existed as a monomer and had a molecular weight of 27 kDa. Moreover, It was very interesting that ?-amylase inhibitor from the mungbean seed inhibited C. maculatus ?-amylase but did not inhibit human salivary ?-amylase. 2D gel electrophoresis suggested that the gamma-induced mutant line has decreased expression of ?-amylase inhibitor and other proteins comparing with its wild type. |
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Researcherดร. อินทิรา ขูดแก้ว, รองศาสตราจารย์ที่ทำงาน:ภาควิชาวิทยาศาสตร์และนวัตกรรมชีวภาพ คณะศิลปศาสตร์และวิทยาศาสตร์ สาขาที่สนใจ:สรีรวิทยา Plant Physiology, ความเครียดของพืช Plant Stress, พฤกษเคมี Phytochemistry, สารทุติยภูมิ Secondary metabolites Resume |